RE: help with transferring plastic sections

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From:jim <>
To:'Gary Radice' <>, HistoNet Server <>
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Assuming that you are floating the sections on water. Make a 3mm loop using 
0.2mm diameter wire. Mount (sticky tape or drop of araldite) the loop's wire 
handle to a "handle". Test that the loop holds a drop of water. When the handle 
is held like a pencil the loop should be horizontal. You could also purchase a 
"perfect loop" from several supplier, including PST.

Insert the loop below the water at the back of the knife's trough. Position the 
loop horizontally below a (or group of) section and lift. The loop should 
contain a drop of water with the section(s). Mark with a felt-tip pen a 5mm 
circle in the middle but the underside of the slide. This circle makes it 
easier to find the sections when staining and later under the scope. Touch the 
loop in the centre and on the up-side of the slide, making a tiny circular 
motion to help release the section with the drop.

Heat the section on a 60 C hotplate for at least 10 minutes before staining or 
heat the section above a spirit flame a little past the drying point.
I like 1.5 um thickness sections for OM best,; they seem a good compromise 
between contrast/staining and sharpness.
Jim Darley
ProSciTech                 Microscopy PLUS
PO Box 111, Thuringowa  QLD  4817  Australia
Ph +61 7 4774 0370  Fax:+61 7 4789 2313
Great microscopy catalogue, 500 Links, MSDS, User Notes

On Tuesday, March 28, 2000 3:02 AM, Gary Radice [] 
> We are cutting glycolmethacrylate sections, around 1 micrometer thick, with
> glass knives and are having a difficult time transfering sections to
> microscope slides without the sections folding. How do you do it/ Does
> anyone have a favorite technique they would care to share?
> Gary P. Radice
> Associate Professor of Biology		804 289 8107 (voice)
> University of Richmond		804 289 8233 (FAX)
> Richmond VA 23173

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