Re: help with transfering plastic sections

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From:Jochen Schuck <jschuck@otogene.de>
To:histonet@pathology.swmed.edu
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Hi Gary,
we have been using Technovit 8100, which is to my knowledge a
glycolmethacrylate and the rolling and folding of thinner sections was
indeed a problem. We solved it by taking the section during cutting
immediately from the knive with tweezers.  We then hold the section over a
waterbath (40-60C) and let it fall onto the water from a few inches. If you
try to place it on the watersurface directly it will usually fold around the
tip of your tweezers.
If you have an automatic microtome then you will find a certain cutting
velocity usefull for getting the rythm of cutting, taking and throwing the
sections.
Watch out though! Be carefull not to let the tip of you tweezers be drawn
into the knive by the block.
Good luck and safe cutting!

Jochen Schuck

> Von: Gary Radice <gradice@richmond.edu>
> Antworten an: histonet@pathology.swmed.edu
> Datum: Mon, 27 Mar 2000 12:02:03 -0500
> An: HistoNet Server <histonet@pathology.swmed.edu>
> Betreff: help with transfering plastic sections
> 
> We are cutting glycolmethacrylate sections, around 1 micrometer thick, with
> glass knives and are having a difficult time transfering sections to
> microscope slides without the sections folding. How do you do it/ Does
> anyone have a favorite technique they would care to share?
> 
> Gary P. Radice   gradice@richmond.edu
> Associate Professor of Biology  804 289 8107 (voice)
> University of Richmond  804 289 8233 (FAX)
> Richmond VA 23173  http://www.science.richmond.edu/~radice
> 
> 
> 




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