Nuclear Staining

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To:'HistoNet' <>

Please forgive me if I am going over old discussions.

We are having a problem with our nuclear staining.  It especially occurs on
tiny biopsies and on lymphoid tissue.  It is that hazy washed out nuclear
pattern.  We have tried about everything to correct the problem.  One our
paths is adamant that it is a heat artifact, I am leaning to incomplete
dehydration.  We have no heat above 63 C. in any of our procedure, we
dehydrate in ethanol and clear in a xylene substitute called Clear-Rite III.
This artifact in intermittent and does not affect all tissues on the run.
It appears to get worse towards the bottom of the slides.  VERY FRUSTRATING!

If anyone has any suggestions on how to correct this please let me know.
Thanks everyone.

Robert M. Sala M.T.(ASCP)
Histology Supervisor
Sioux Valley Hospital
1100 S. Euclid Ave.
Sioux Falls, SD  57117-5039

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