Re: Hirsch-Pfieffer
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From: | RSRICHMOND@aol.com |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="US-ASCII" |
Dealing with a single case of metachromatic leukodystrophy - a sural nerve
biopsy specimen - about thirty years ago - I found the literature about the
"brown metachromasia" of the cerebroside sulfatide storage product (in this
dismal inherited disease) to be extremely unsatisfactory - and I can read
German.
A simple toluidine blue stain sufficed to demonstrate it, as I remember, but
mounting was a problem. Going directly through water to dioxane, with a
homemade mounting medium of polystyrene dissolved in dioxane, was what we did
at the time. (Dioxane has a reputation for toxicity that probably precludes
its use today.)
The brown metachromasia lasted for about a day. I believe that the only
permanent medium for brown metachromasia is photographic film!
Bob Richmond
Samurai Pathologist
Knoxville TN
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