References for cryostat decontamination

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From:Tim Morken <>
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Cryostat decontamination references:

College of American Pathologists, Commentary on certification questionaire:
1) Decontaminate regularly with 70% alcohol.
2) Defrost, remove trimmings and decontaminate with a tuberculocidal 
disinfectant, preferably weekly for instruments used daily
3) Reference: National Committee for Clinical Laboratory Standards (NCCLS), 
Protection of Laboratory Workers from Instrument Biohazards and Infectious 
Disease Transmitted by Blood, body Fluids, and Tissue; Approved Guideline 
M29-A. Wayne, PA, NCCLS, 1997

CAP site:

NCCLS site:
Seach for "instrument biohazards" and you will get a list of publications 
that address various hazards.

Decontamination for HIV
Frozen Section Technique for Tissues Infected by the AIDS Virus, Swisher, 
B.L., Ewing, E.P., J Histotechnol, Vol.9, No.1, p.29 (March 1986)
Recommends 95% ETOH (other publications referenced here indicate 95% alcohol 
is effective against HIV).

Department of Agriculture for Northern Ireland, Safe Working and the 
Prevention of Infection in Clinical Laboratories, Health Services Advisory 
Recommends formalin fuming for 24-48 hours at room temperature. Followed by 
ammonia for one hour (presumably as a neutralizer).

Laboratory Histopathology: A Complete Reference, Woods and Ellis, Churchill 
Livingstone, 1994.
Recommends defrosting , washing with 70% alcohol, complete cleaning.

For those who like to read a lot:
APIC Guideline for Selection and Use of Disinfectants, Association for 
Professionals in Infection Control (APIC), American Journal of Infection 
Control, Vol 24(4), August 1996, 313-342. A good review of all disinfectants 
in use and the pros and cons of each.

Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
1600 Clifton Rd.
Atlanta, GA 30333


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