Re: aptes coating for in situ hyb.
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From: | Tony Henwood <henwood@mail.one.net.au> |
To: | histonet@pathology.swmed.edu, "Marshall, Sharon," <marshall@anat.uct.ac.za> |
Reply-To: | |
Content-Type: | text/plain; charset=US-ASCII |
Dear Sharon,
Re:
> I would like to know what special procedures, if any, are needed for
> coating slides for in situ with aptes. We have been using aptes in
> the same way we use it for ordinary light microscopy sections but I
> am now wondering if we should not rather change this. If someone
> who does in situ often could e-mail us their aptes procedure that
> would be great.
You might find the info you need on the Homepages below (one.net.au)
in summary, here it is:
The method of Burns etal (J.Clin.Pathol., 40:858-864, 1987) works quite well:
Wash slides in 2% Decon (BDH 56042), 10 minutes.
Rinse slides in water, 3 minutes.
Rinse in acetone, 1 minute.
Immerse in 2% APTS (Sigma A3648) in acetone, 5 minutes.
Wash in water, 2 minutes.
Dry at room temperature overnight.
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital
Sydney, AUSTRALIA
http://www2.one.net.au/~henwood
http://www.pathsearch.com/homepages/TonyHenwood/default.html
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