Re: aptes coating for in situ hyb.

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From:Tony Henwood <>, "Marshall, Sharon," <>
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Dear Sharon,

> I would like to know what special procedures, if any, are needed for 
> coating slides for in situ with aptes.  We have been using aptes in 
> the same way we use it for ordinary light microscopy sections but I 
> am now wondering if we should not rather change this.  If  someone 
> who does in situ often could e-mail us their aptes procedure that 
> would be great.

You might find the info you need on the Homepages below ( 
in summary, here it is:

The method of Burns etal (J.Clin.Pathol., 40:858-864, 1987) works quite well: 

Wash slides in 2% Decon (BDH 56042), 10 minutes. 
Rinse slides in water, 3 minutes. 
Rinse in acetone, 1 minute. 
Immerse in 2% APTS (Sigma A3648) in acetone, 5 minutes. 
Wash in water, 2 minutes. 
Dry at room temperature overnight. 

Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital

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