-----Original Message-----
From: Pam Marcum [mailto:pmarcum@polysciences.com]
Sent: Monday, March 13, 2000 8:35 AM
To: amos brooks; DENISE BLAND-PIONTEK
Cc: histonet@pathology.swmed.edu
Subject: RE: Daily Digest


It depends on the pH of the tap water and the contaminants therein.
Different areas may have very different stain reactions to tap water.  We
have very basic tap water and some things I did before will not work here
the same way.  The pH there was more acidic.  Pam Marcum

-----Original Message-----
From: amos brooks [mailto:atbrooks@snet.net]
Sent: Thursday, March 09, 2000 3:58 PM
To: DENISE BLAND-PIONTEK
Cc: histonet@pathology.swmed.edu
Subject: Re: Daily Digest


Hi,
    Three questions about this:
    1) When you are preparing the mucicarmine solution (diluting the stock)
are you using tap water or distilled? For some reason the tap water works
better, I dont know why. (If someone could enlighten me I'd be thrilled)
    2) Is there any autolysis in the epithelium of this stomach specimen?
Sometimes the goblet cells are the first to be affected.
    3) What part of the stomach are you staining? Some parts are less
reactive than others.
Amos Brooks

DENISE BLAND-PIONTEK wrote:

> RE:MUCICARMINE
>
> Dear Histonetters:
>      I have questions regarding a mucicarmine stain being done on distal
stomach.
> The stain has been done on different sections of stomach, distal to insure
mucin, but it is not turning out. We have checked the chemicals, expiration
dates (etc.). The same recipe and solutions have worked on other types of
tissue. Is there a specific reason why stomach is resistant to mucicarmine?
Any suggestions are appreciated.
>
> Thankyou,
> Denise Bland-Piontek, HTL(ASCP)
>
> >>> HistoNet Server <histonet@pathology.swmed.edu> 03/04/00 11:00PM >>>
>
> ----------------------------------------------------------------------
>
> Date: 4 Mar 2000 03:45:17 -0600
> From: "Tony Henwood" <henwood@mail.one.net.au>
> Subject: Re:
>
> Dear Marianne,
> Would someone tell me, my gold chloride made in triple distilled water
> froze, is it still good to use?
>
"Daily Digest a.k.a. MUCICARMINE". I did not realize this was actually a
thread.  I would have missed Amos's reply altogether if Pam's reply had not
accidentally opened. Please when you reply to a post and you subscribe to
the Daily Digest change the subject line to match the topic. Thank you. maj