CJD Question.

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From:"Johnson, Mickey" <JohnsoM@shmc.org>
To:"'HistoNet Server' (E-mail)" <HistoNet@Pathology.swmed.edu>
Content-Type:text/plain; charset="iso-8859-1"

Hi Histonetters!

I know this subject has been discussed to death. The recent info about
fixation with Formalin followed by Formic Acid, etc has been very helpful.
The question I have is:  What do you do to decontaminate a cryostat when a
frozen has been done on a suspected CJD case?  With the new electronically
controlled cryostats, it is impossible to decontaminate completely without
damaging the electronic components. I have used alcohol to wipe up the
inside and used straight bleach on the knife holder and other removable
components, and then used straight bleach on the inside walls that I could
reach.   What I am interested in is whether your lab has any policy
regarding NOT doing frozens on CJD (or suspected) cases? We have such a
policy for autopsies, because it is impossible for us to contain the bone
dust when opening the skull to remove the brain.  Our epidemiologist and I
are going to write a hospital policy about frozen sections and could really
use input from any and all of you.  Thank you for help on this. 

Best Regards.


Michael L Johnson, BS, HTL(ASCP)
Histology Supervisor
Department of Pathology
Sacred Heart Medical Center
W. 101 8th Avenue
Spokane, WA 99220


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