Joanne,
when I was at the AFIP in Washington D.C., we had problems with the heat and
humidity in the lab.  We experienced severe background staining.  We had to
decrease the incubation times of all the antibodies.  Since the air handler
often broke during the summer, we became adept at figuring out what to do.
We usually cut all the incubation times by 5 minutes.  At that time, all our
immuno were performed manually.  Hope this helps.

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas 


> -----Original Message-----
> From:	Suder, Joanne [SMTP:JC_Suder@fccc.edu]
> Sent:	Monday, June 07, 1999 3:20 PM
> To:	'HISTONET@PATHOLOGY.SWMED.EDU'
> Subject:	heat and humididty
> 
> Histonetters,
>   I am searching for any references discussing the affects of room
> temperature and humidity on immuno staining. Our room temp and humidity
> functuate constantly and we can see a variation in our staining from one
> day
> to the next.
> 
> Thanks