At our old site we also experienced some fluctuation in immuno staining. It
certainly appeared to be due to changes in temp and humidity in the room as
environmental conditions were poorly controlled (no air con in summer, do
humidification in winter) and the fluctuations seemed to be somewhat
seasonal. We are now at a temp and humidity controlled site, and staining
seems to be more consistent.
b 

> -----Original Message-----
> From:	Suder, Joanne [SMTP:JC_Suder@fccc.edu]
> Sent:	Monday, June 07, 1999 4:20 PM
> To:	'HISTONET@PATHOLOGY.SWMED.EDU'
> Subject:	heat and humididty
> 
> Histonetters,
>   I am searching for any references discussing the affects of room
> temperature and humidity on immuno staining. Our room temp and humidity
> functuate constantly and we can see a variation in our staining from one
> day
> to the next.
> 
> Thanks