RE: brdu in decalcified bone?

From:Elizabeth Chlipala


We have frequently used 5% formic acid decalcified mouse bone for
demonstration of BrdU without any lose of staining.  We fixed in zinc
formalin for no longer than 48 hours and decaled in 5% formic acid.


Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
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University of Colorado
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-----Original Message-----
From: Garlits, John [] 
Sent: Tuesday, June 03, 2003 11:47 AM
To: HistoNet Server
Subject: brdu in decalcified bone?


I am writing to ask if anybody has experience using BRDU in decalcified
bone.  We typically use acid decalcification of formalin-fixed mouse
bone.  I had heard that the decalcification could cause problems for
subsequent BRDU immunohistochemistry.  Is it necessary to use EDTA


John Garlits, M.S.
Senior Research Technician
Hematology Oncology Division
Experimental Hematology Department
St. Jude Children's Research Hospital
332 N Lauderdale
Memphis, TN 38108
Phone: 901-495-3961
Fax: 901-495-2176

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