RE: brdu in decalcified bone?
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
From: Elizabeth Chlipala [mailto:firstname.lastname@example.org]
Sent: Tuesday, June 03, 2003 2:05 PM
To: 'Garlits, John'; 'HistoNet Server'
Subject: RE: brdu in decalcified bone?
We have frequently used 5% formic acid decalcified mouse bone for
demonstration of BrdU without any lose of staining. We fixed in zinc
formalin for no longer than 48 hours and decaled in 5% formic acid.
Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Histology Laboratory
University of Colorado
MCBD, Room A3B40
Boulder, Colorado 80309
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From: Garlits, John [mailto:John.Garlits@stjude.org]
Sent: Tuesday, June 03, 2003 11:47 AM
To: HistoNet Server
Subject: brdu in decalcified bone?
I am writing to ask if anybody has experience using BRDU in decalcified
bone. We typically use acid decalcification of formalin-fixed mouse
bone. I had heard that the decalcification could cause problems for
subsequent BRDU immunohistochemistry. Is it necessary to use EDTA
John Garlits, M.S.
Senior Research Technician
Hematology Oncology Division
Experimental Hematology Department
St. Jude Children's Research Hospital
332 N Lauderdale
Memphis, TN 38108
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