cryostat works great..thanks everyone
Thnak you to all who sent advice about using the cryostat. I decided not to
be afraid of it and took the microtome out and cleaned it and reassembled it
with some oil. It was obviously installed improperly before, now that it is
back together it works great. I cut some nice unwrinkled 5um sections :)
If anyone has experience with ovarian tumours it would be good to contact
you. I am wondering what tempurature to cut them at (although -19 yesterday
seemed to work very nice) or anything else I might need to know. I am also
wondering if anyone has any experience staining slides that won't be index
matched. It is difficult to see good morphology without the cover slip.
Thanks again.
Jason.
ICM.
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