RE: HE stain without Blue reagent

From:

"Smith, Allen"

Yes. I would add a few milligrams of sodium carbonate or bicarbonate instead of sodium hydroxide. In most places in the world, the tap water itself will blue hematoxylin without added base.

Allen A. Smith, Ph.D.
Barry University
School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161-6695

-----Original Message-----
From: yan gao [mailto:gliuygao@hotmail.com]
Sent: Thursday, June 05, 2003 10:55 AM
To: histonet@pathology.swmed.edu
Cc: gliuygao@hotmail.com
Subject: HE stain without Blue reagent

Hi, Histonet. Very simple question. My lab. run out Blue reagent. If I want to H&Estain, can I get good stain just run tape warm water by add one drop sodium Hydroxide? Thanks.

Yan

Add photos to your e-mail with MSN 8. Get 2 months FREE*.

The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses.
Barry University - Miami Shores, FL (http://www.barry.edu)

<< Previous Message | Next Message >>