RE: HE stain without Blue reagent
Yes. I would add a few milligrams of sodium carbonate or
bicarbonate instead of sodium hydroxide. In most places in the world, the
tap water itself will blue hematoxylin without added base.
Allen A. Smith, Ph.D.
School of Graduate
Podiatric Medicine and Surgery
Shores, Florida 33161-6695
Hi, Histonet. Very simple question. My lab. run out Blue
reagent. If I want to H&Estain, can I get good stain just run
tape warm water by add one drop sodium Hydroxide? Thanks.
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