RE: HE stain without Blue reagent

From:"Smith, Allen"

Yes.  I would add a few milligrams of sodium carbonate or bicarbonate instead of sodium hydroxide.  In most places in the world, the tap water itself will blue hematoxylin without added base.

Allen A. Smith, Ph.D.
Barry University
School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161-6695

-----Original Message-----
From: yan gao []
Sent: Thursday, June 05, 2003 10:55 AM
Subject: HE stain without Blue reagent

Hi, Histonet.  Very simple question.  My lab. run out Blue reagent.  If I want to H&Estain,  can I get good stain just run tape warm water by add one drop sodium Hydroxide?  Thanks.

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