RE: HE stain without Blue reagent

From:Tony Henwood

Any slightly alkaline aqueous  solution will do. A few drops of ammonia in water or 1/10 solution of saturated lithium carbonate for example.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: yan gao []
Sent: Friday, 6 June 2003 0:55
Subject: HE stain without Blue reagent

Hi, Histonet.  Very simple question.  My lab. run out Blue reagent.  If I want to H&Estain,  can I get good stain just run tape warm water by add one drop sodium Hydroxide?  Thanks.

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