Re: Where to get alcian blue from? Where are the squames comeing from

From:Roger Moretz


Here is one supplier for alcian blue:
EM Sciences:
None of the other EM suppliers have it listed anymore.
 There has been a lot of postings on histonet about
the unavailability of alcian blue.  A quick review of
the archives
( should give
you the information you need.

Roger Moretz, Ph.D.
Dept of Toxicology
BI Pharmaceuticals, Inc.
Rigdefield, CT

--- kevin williams  wrote:

Dear all, lots of questions,
Where can I buy Alcian Blue. No one seems to have it
in there catalog. I dont want the stianing kit, I
recon I can do it for less with the dry powder, it
dose come in a dry powder? 
Secondly we have been getting squames coming onto the
slides that go through our linistain ( belt chain )
stainer, I love simplicity,  I recon they must come
from me becaues I am the only one cutting etc. So any
way to stop this. We use normal water, not distilled
in our water bath and I do not wear gloves any ideas. 
Also our Amoium Hydroxide, we put about 0.6 ml
concentrated, straight from the bottle,  into about
120ml of distilled H2O. We did use about 1ml but
sections keep comeing off, but now we have background
on the slides. Can I use more stay on, and we do use
tap water in the water bath, or should I just keep
trying to increase the ammonium hydroxide
concentration a little bit more. Altready though the
sections start to fold and flap as parts begin to come
free of the slide. The result looks just as bad.
Finally is there anyone from the Vermont and New
Hampshire Histology Society. I am interested in
joining. All the best and thanks. 
A. Kevin Williams

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