RE: decalcification in EDTA and nitric acid

From:Carl Hobbs

My tuppence worth.......decal in EDTA at 37C ( change regularly as EDTA
chelates Ca++)and use Xray or a chem test, once you've liberatred the Ca++
from EDTA ( hence "sequestrene") OR use Formic acid or, better,
Formic-formate and chem test for Ca or Xray. If it's purely for histological
examination, mineral acids are fine but use at5%. We always found it better,
where possible, to bisect the tooth longitudinally , before decal, using a
special cutting apparatus but.....hey! that costs.Any problems we had were
always found to be due to inadequate processing or blunt knives.
Carl Hobbs
Laboratory Manager
Molecular Neurobiology Group
MRC Centre For Developmental Neurobiology
New Hunts House
Guys Campus
Kings College London
020 7848 6810/fax 6816

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