Help with a hands-on workshop on IHC
Dear IHC "guru's",
We have the pleasure of working with 16 college students this
summer who are here for an NSF - funded research experience. I have
already done one three hour workshop on microscopy/imaging techniques and
another three hour on histology where we had the paraffin ribbon cutting
contest with prizes awarded for the longest paraffin ribbon!
Now, the faculty would like us to teach a hands-on workshop on
IHC. Since I have limited experience in this area, I would like some
suggestions. The theory/lecture part is no problem. However, the hands-on
portion is where I would like some input.
1. What kit should I purchase (they will be working in groups of 4)?
2. What tissue should I use...species, etc. ( I can obtain human, bovine,
porcine, rat, and mice tissue)
We need something simple, easily reproduced, and relatively
inexpensive. I guess my question to the expert's is...if you had to
showcase your favorite IHC stain what would it be and why?
Many, many years ago, I remember the first time I did a Masson's
Trichrome stain and thought it was the most beautiful sight to
behold...still my favorite "special stain"!
My sincere thanks in advance,
Linda Jenkins, HT
Dept. of Bioengineering
Clemson, SC 29634-0905
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