I only use PLL-coated slides for IHC.  I coat my own, purchasing the stock
PLL from Sigma and diluting it out.  Much cheaper than buying (+)-charged
slides.

Jan Shivers
  ----- Original Message -----
  From: Agustín Venzano
  To: HistoNet Server
  Sent: Friday, June 28, 2002 9:20 AM
  Subject: Poly-L-Lysine


  Hello all netters! Have all of you switched from Poly-L-Lysine- coated
slides to positively-cahrged ones? Or is anyone still using the classic PLL-
coated slides for IHC? Is it still worth considering this possibility? What
was your experience on the subject?

  Thank you in advance

  Agustin Jose venzano
  DVM- INTA Castelar, Argentina