Re: CD31 in FFPE mouse tissues

From:rueggp

I forgot to mention that the anti-human CD 31 I used from Research Diagnostics
worked on zffpe rat tissue as well as the anti-rat cd31.
patsy

Luis Chiriboga wrote:

> Do you recall the clone ??
>
> -----Original Message-----
> From: r-meyer2@northwestern.edu [mailto:r-meyer2@northwestern.edu]
> Sent: Friday, June 28, 2002 8:40 AM
> To: histonet@pathology.swmed.edu
> Subject: Re: CD31 in FFPE mouse tissues
>
> Teri,
>
> I just completed a project usind CD31 on FFPE mouse tissue with good
> results.
> The supplier of the antibody is Research Diagnostics (973-584-7093;
> www.researchd.com).  I used rat anti-mouse CD31, monoclonal; Catalog#
> RDI-mCD31abrt.  They also suggested zinc fixation, but it worked on formalin
> fixation.  I used DAKO's Prot K to open up the sights.
>
> Bob Meyer
> Pathology Core Facility
> Northwestern University
>
> >
> > I'm looking for a supplier of CD31 to stain formalin-fixed, paraffin
> embedded
> mouse tissue.  I understand some companies offer antibodies that don't work
> well in formalin fixed material, but instead suggest using zinc fixed tissue
> (not zinc formalin fixed).  Unfortunately, I don't happen to have any zinc
> fixed tissue at the moment.
> >
> > Thanks in advance!
> >
> > Teri Johnson
> > Managing Director Histology Core Facility
> > Stowers Institute for Medical Research
> > 1000 E. 50th St.
> > Kansas City, Missouri  64110
> > tjj@stowers-institute.org
> >
> >





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