Fernando Capela e Silva wrote:
> I would like someone send me information about the concentration of Calcein
> (gr to 100 ml) I must use to inject in the animals and the amount (ml) of this
> solution/injection/animal

Dear Fernando,
If someone answers your Histonet question with a 
concentration and a dose, with a reference to a
paper in a peer-reviewed journal, you should NOT
go ahead and follow the advice.  You must find
the paper and read it carefully.

You are doing RESEARCH, and the only way for
you (or your supervisor) to obtain the information
is from reading the original peer-reviewed papers
in the field. A Histonet reply is not peer-reviewed
and it could be Wrong+++.  Lots are.

Even the peer-reviewed literature must be read 
critically and with a suspicious and cynical 
attitude. As one of my students told me more than 
30 years ago, "The literature is full of lies." 
He was a bit younger and a lot wiser than I, but 
I have known for about 25 years that he was Right+++.

The peer-review system is the only way to police
scientific publication, but plenty of bad papers
get published. An item that has not been 
peer-reviewed is worthless and has an 
above-average chance of being either ignorantly
or maliciously untrue. You cannot do serious
work (such as diagnosis or research) on the basis
of internet gossip.
-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan@uwo.ca
   http://publish.uwo.ca/~jkiernan/