Cracked Liver Sections

From:Tracey Gunn

Hi All,

We are currently sectioning and H&E staining 600 paraffin embedded sheep
liver blocks for a client.  They have been fixed in 10% NBF.  We are having
terrible trouble with the tissue sections cracking after being mounted on
the slides.  We have tried floating out on tepid waterbaths, soaking in
iced water, cutting the blocks at room temperature and to no avail.  Any
ideas before I become bald from pulling my hair out?

Tracey Gunn.

Tracey Gunn
Histology Service Unit
University of Otago
New Zealand

Phone: (03) 479 7152
Fax: (03) 479 7136

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