Re: toxicity in brain

From:Cathy Gorrie

Frozen sections can be cut and stained with H&E for immediate
diagnostic reasons, ie resection lines for melanoma lesions. I don't
have the exact procedure on hand but the slide mounted sections are
fixed in acetone? if I remember right and then a rapid H&E is
performed. I'm sure someone will have a protocol. The morphology
isn't ever going to be as good as well fixed tissue but if
pathologists can diagnose from these, you should be able to see what
you're looking for.

I find a cresyl violet stain is better for brain tissue especially if
the sections are reasonably thick (30-50Ám), but it depends what
you're interested in, and I don't know how well it will work on post
fixed sections.


At 3:39 PM -0400 6/6/02, wrote:
>I hope you folks will forgive a naive question from a psychologist who does
>some histology & is trying to help another psychologist who knows even less
>about histology:  she wants to use H&E to assess toxicity in rat brain, but
>in fresh-frozen brain (not yet sectioned).  I've provided her with a
>protocol for H&E, but can the slide-mounted sx's be fixed adequately at that
>stage for H&E to work?  Or might there be another stain or assay that would
>work better for this purpose?  Thanks for any advice!   Susan

Cathy Gorrie
Scientific Officer
Neural Injury Research Unit,
School of Medical Sciences,
University of New South Wales
Sydney, N.S.W. 2052

Phone: 61-2-9385 2462
Fax   : 61-2-9313 6252

<< Previous Message | Next Message >>