Yepper to dehydration..Throw your blocks back in some ice water for a min or
2..Then proceed..Do it between levels...Works like a charm!
----- Original Message -----
From: "K C" 
To: "Histonet" 
Sent: Wednesday, June 05, 2002 7:40 PM
Subject: GI biopsies


> I have currently been doing some routine H&E staining
> of GI biopsies on paraffin sections and have recently
> come upon a problem.  When cutting different levels,
> up to 5 levels, I notice a progressive, what appears
> to be, tearing of the tissue.  The first level comes
> out perfect and as I go through levels, the "tears"
> seem to be worse.
>
> We have tried different microtomes, different knives,
> and also different people cutting, with the same
> result.
>
> Also, we have kept the blocks cold until cut.
>
> The funny thing is, you can't pick the day this will
> happen.  Some days, the sections are perfect....other
> days we get this phenomenon.
>
> Anyone out in Histoland have any ideas?
>
> John
>
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