RE: Tarpley snap freezing with dry ice/isopentane

From:"Tarpley, John" <>

We do usually use Tissue Tek Cryomolds, but for samples larger than the
molds we have used PeelAway molds. However, remember that the larger the
sample the slower the freeze and the greater the chance for artifacts.

John E. Tarpley 5-1-A
Associate Scientist
Amgen Inc.
One Amgen Center Drive
Thousand Oaks, CA 91320 
These Opinions are my own and not necessarily those of my employer.

-----Original Message-----
From: Gayle Callis []
Sent: Wednesday, June 27, 2001 8:38 AM
Subject: Tarpley snap freezing with dry ice/isopentane

Brain snap freezing has been discussed a great deal recently.  John Tarpley
gave an excellent explanation on how to snap freeze tissues using
cryomolds, OCT and a dry ice/isopentane slurry method.  He does NOT get
cracked blocks, and it would worth a try for those having problems. John
did not give brand of cryomold he used, but we have found Tissue Tek
cryomolds superior due to thinner plastic in construction.  This may not be
a factor with John's method but you can ask him. 

 This was published in Microscopy Today, June 2001, Issue #01-5.  Maybe
John would drop a file attachment to you IF you cannot access the journal.

John can be contacted at and is willing to help you out. 


Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610

406 994-6367
404 994-4303 (FAX)

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