RE: Whole body autoradiography
|From:||Abizar Lakdawalla <firstname.lastname@example.org>|
Hi Gayle, can the cryostat cut through the bones of an intact animal?
> -----Original Message-----
> From: Gayle Callis [mailto:email@example.com]
> Sent: Wednesday, June 27, 2001 7:56 AM
> To: firstname.lastname@example.org
> Subject: Re: Whole body autoradiography
> We looked into this last year. A Leica sales rep, a lady, gave me the name
> of a company who could whole mouse frozen sections (not autoradiography).
> After pricing Leica macrocryostat to the tune of $150,000 plus that takes
> up vast lab space, we tried finding a contract lab. Now here is the bad
> news, this would have cost us something like $20,000/mouse
> (probably more!)
> for serial sections. You can call Leica for information on purchasing a
> large cryostat, and reach her. She should be able to come up with a
> contract lab. Sorry, don't have her name handy.
> There is a group in Minnesota who tracked injected enzyme
> labelled cells in
> a live mouse - not sure exactly how they did this, antibody? onto
> (probably could do enzyme conj antibodies/your protocol) inject a
> substrate that lights up, a chemiluminescent endpoint, and view the whole
> body with a CCD camera. The camera was probably cheaper than big cryostat
> OR contract work, plus you could probably do more experimentation with the
> animal. It was totally elegant as is many modern technology technics! We
> are still considering a camera.
> Good luck.
> Gayle Callis
> Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> Bozeman MT 59717-3610
> 406 994-6367
> 404 994-4303 (FAX)
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