RE: Whole body autoradiography

From:"Tarpley, John" <>

<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN"> <HTML><HEAD> <META HTTP-EQUIV="Content-Type" CONTENT="text/html; charset=iso-8859-1"> <META content="MSHTML 5.50.4134.600" name=GENERATOR></HEAD> <BODY> <DIV><SPAN class=067013418-27062001><FONT face=Arial color=#0000ff size=2>Having absolutely nothing against the fine cryostats made by Bright for Hacker, I do want to mention that if you choose that instrument over a sliding type microtome present in the macrocryostat mentioned in Gayle's post you will be limited to smaller animals. While this may not pose a problem you should think about the size mouse or rat to be sectioned prior to purchasing equipment.</FONT></SPAN></DIV> <DIV><SPAN class=067013418-27062001><FONT face=Arial color=#0000ff size=2></FONT></SPAN> </DIV> <DIV><SPAN class=067013418-27062001> <P><FONT size=2>John E. Tarpley 5-1-A<BR>Associate Scientist<BR>Amgen Inc.<BR>One Amgen Center Drive<BR>Thousand Oaks, CA 91320<BR>These Opinions are my own and not necessarily those of my employer.</FONT> </P></SPAN></DIV> <BLOCKQUOTE> <DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma size=2>-----Original Message-----<BR><B>From:</B> []<BR><B>Sent:</B> Wednesday, June 27, 2001 9:28 AM<BR><B>To:</B><BR><B>Cc:</B><BR><B>Subject:</B> Re: Whole body autoradiography<BR><BR></FONT></DIV><FONT face=arial,helvetica><FONT size=2>As an FYI, I would like to call to your attention that whole body <BR>autoradiography on mice can be performed without any difficulty on the <BR>Hacker-Bright OTF with large area modification. This has been done, I venture <BR>to say, for decades on this instrument. The cost point is <B><U>well under </B></U>$30,000, <BR>so anyone considering this procedure should not be misled to believe that it <BR>involves such as massive investment. <BR><BR>If you'd like more information, please feel free to give us a call or send an <BR>e-mail. Dorothy Murphy is the best person here to speak with about details of <BR>the procedure. <BR><BR>Best regards, <BR><BR>Elfi Hacker <BR>HACKER Instruments & Industries Inc <BR>1-800-4-HACKER <BR><BR> <BLOCKQUOTE style="PADDING-LEFT: 5px; MARGIN-LEFT: 5px; BORDER-LEFT: #0000ff 2px solid; MARGIN-RIGHT: 0px" TYPE="CITE">We looked into this last year. A Leica sales rep, a lady, gave me the name <BR>of a company who could whole mouse frozen sections (not autoradiography). <BR>After pricing Leica macrocryostat to the tune of $150,000 plus that takes <BR>up vast lab space, we tried finding a contract lab. Now here is the bad <BR>news, this would have cost us something like $20,000/mouse (probably more!) <BR>for serial sections.  You can call Leica for information on purchasing a <BR>large cryostat, and reach her.  She should be able to come up with a <BR>contract lab.  Sorry, don't have her name handy. <BR><BR>There is a group in Minnesota who tracked injected enzyme labelled cells in <BR>a live mouse - not sure exactly how they did this, antibody? onto CD4/CD8?? <BR>(probably could do enzyme conj antibodies/your protocol) inject a <BR>substrate that lights up, a chemiluminescent endpoint, and view the whole <BR>body with a CCD camera.  The camera was probably cheaper than big cryostat <BR>OR contract work, plus you could probably do more experimentation with the <BR>animal.  It was totally elegant as is many modern technology technics! We <BR>are still considering a camera. <BR><BR>Good luck. <BR><BR><BR>Gayle Callis <BR>MT,HT,HTL(ASCP) <BR>Histopathology Supervisor <BR>Veterinary Molecular Biology <BR>Montana State University - Bozeman <BR>Bozeman MT 59717-3610 <BR><BR>406 994-6367 <BR>404 994-4303 (FAX) <BR><BR><BR></BLOCKQUOTE><BR><BR></BLOCKQUOTE></FONT></FONT></BODY></HTML>
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