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<DIV><SPAN class=067013418-27062001><FONT face=Arial color=#0000ff size=2>Having
absolutely nothing against the fine cryostats made by Bright for Hacker, I do
want to mention that if you choose that instrument over a sliding type microtome
present in the macrocryostat mentioned in Gayle's post you will be limited to
smaller animals. While this may not pose a problem you should think about the
size mouse or rat to be sectioned prior to purchasing
equipment.</FONT></SPAN></DIV>
<DIV><SPAN class=067013418-27062001><FONT face=Arial color=#0000ff
size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=067013418-27062001>
<P><FONT size=2>John E. Tarpley 5-1-A<BR>Associate Scientist<BR>Amgen
Inc.<BR>One Amgen Center Drive<BR>Thousand Oaks, CA 91320<BR>These Opinions are
my own and not necessarily those of my employer.</FONT> </P></SPAN></DIV>
<BLOCKQUOTE>
<DIV class=OutlookMessageHeader dir=ltr align=left><FONT face=Tahoma
size=2>-----Original Message-----<BR><B>From:</B> HACKERLAB@aol.com
[mailto:HACKERLAB@aol.com]<BR><B>Sent:</B> Wednesday, June 27, 2001 9:28
AM<BR><B>To:</B> uvsgc@montana.edu<BR><B>Cc:</B>
histonet@pathology.swmed.edu<BR><B>Subject:</B> Re: Whole body
autoradiography<BR><BR></FONT></DIV><FONT face=arial,helvetica><FONT size=2>As
an FYI, I would like to call to your attention that whole body
<BR>autoradiography on mice can be performed without any difficulty on the
<BR>Hacker-Bright OTF with large area modification. This has been done, I
venture <BR>to say, for decades on this instrument. The cost point is
<B><U>well under </B></U>$30,000, <BR>so anyone considering this procedure
should not be misled to believe that it <BR>involves such as massive
investment. <BR><BR>If you'd like more information, please feel free to give
us a call or send an <BR>e-mail. Dorothy Murphy is the best person here to
speak with about details of <BR>the procedure. <BR><BR>Best regards,
<BR><BR>Elfi Hacker <BR>HACKER Instruments & Industries Inc
<BR>1-800-4-HACKER <BR><BR>
<BLOCKQUOTE
style="PADDING-LEFT: 5px; MARGIN-LEFT: 5px; BORDER-LEFT: #0000ff 2px solid; MARGIN-RIGHT: 0px"
TYPE="CITE">We looked into this last year. A Leica sales rep, a lady, gave
me the name <BR>of a company who could whole mouse frozen sections (not
autoradiography). <BR>After pricing Leica macrocryostat to the tune of
$150,000 plus that takes <BR>up vast lab space, we tried finding a contract
lab. Now here is the bad <BR>news, this would have cost us something like
$20,000/mouse (probably more!) <BR>for serial sections. You can call
Leica for information on purchasing a <BR>large cryostat, and reach her.
She should be able to come up with a <BR>contract lab. Sorry,
don't have her name handy. <BR><BR>There is a group in Minnesota who tracked
injected enzyme labelled cells in <BR>a live mouse - not sure exactly how
they did this, antibody? onto CD4/CD8?? <BR>(probably could do enzyme conj
antibodies/your protocol) inject a <BR>substrate that lights up, a
chemiluminescent endpoint, and view the whole <BR>body with a CCD camera.
The camera was probably cheaper than big cryostat <BR>OR contract
work, plus you could probably do more experimentation with the <BR>animal.
It was totally elegant as is many modern technology technics! We
<BR>are still considering a camera. <BR><BR>Good luck. <BR><BR><BR>Gayle
Callis <BR>MT,HT,HTL(ASCP) <BR>Histopathology Supervisor <BR>Veterinary
Molecular Biology <BR>Montana State University - Bozeman <BR>Bozeman MT
59717-3610 <BR><BR>406 994-6367 <BR>404 994-4303 (FAX)
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