Dear Lester,

	I had similar problems until I worked out that my xylene substitute was
not compatable with my xylene based coverslipping medium.  This seems to be
a problem with some substitutes but not others.

	There are 3 solutions; 1/Dry your slides before you coverslip. They come
out just beautifully.  2/ buy a compatable coverslipping medium.  3/ Use a
different xylene substitute.

	Regards	

	Rob.

At 01:42 AM 7/18/99 -0300, you wrote:
>Hello Members
>
>My H&E slides end up with bubbles between the sections. I wonder if the
>fault lies among the use Superfrost plus (electrostatic) slides or
>xylene substitue or permount, all of which I use. Any experiences here?
>The bubbles may be water but I am not sure.
>Lester


R. Wadley, B.App.Sc. M.L.S, Grad.Dip.Sc.MM
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) 	+61 (2) 9385 3517
Ph (AH)	+61 (2) 9555 1239
Fax 	+61 (2) 9385 1591
E-mail	r.wadley@unsw.edu.au
www	http://www.micro.unsw.edu.au/caf.html