Re: bubbles

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From:"sharon oaborn" <>
To:"Lester C. N. Simon" <>, "Histonet" <>
Date:Sun, 18 Jul 1999 13:16:50 -0700
Content-Type:text/plain; charset="iso-8859-1"

Lester, if the appearance of the slide is milky, you have water still in the
tissues.  Xylene substitutes take longer to remove the alcohols.  The
alcohols need to remove the water.  Try allowing the slide to remain in
fresh alcohols and in fresh xylene substitues a little longer than you are
currently.  Also, your permount may not be compatible with the xylene
substitute.  Check with the substitute manufacturer for compatible mounting
media.   Please make certain the substitute is sheeting over the slide
before placing mounting media on it.  This sheeting action lets us know the
other solvents are removed from the slide.  Place your drop of mounting
media on the lower edge of slide and let it run down the edge.  Apply your
cover slip at a 45 degree angle along the lower edge with the mounting
media.  Allow the coverslip to gently and slowly lay down on the slide as
you rotate it flat.  Capillary action of the mounting media will produce a
smooth air bubble free slip.  You may need to add a slight more mounting
media than you are using if you are unable to gently press on the coverslip
and move the bubble out.  sharon osborn
-----Original Message-----
From: Lester C. N. Simon <>
To: Histonet <>
Date: Saturday, July 17, 1999 11:54 PM
Subject: bubbles

>Hello Members
>My H&E slides end up with bubbles between the sections. I wonder if the
>fault lies among the use Superfrost plus (electrostatic) slides or
>xylene substitue or permount, all of which I use. Any experiences here?
>The bubbles may be water but I am not sure.

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