Harris hematoxylin (safety questionnaire)
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From: | RSRICHMOND@aol.com |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Date: | Sat, 31 Jul 1999 17:28:29 EDT |
Content-Type: | text/plain; charset="us-ascii" |
Ken Urban at Surgipath writes:
>>Surgipath has been producing its version of the Harris hematoxylin since
1980. We have never used mercuric oxide in our formulation. Ingredients are
printed on the label.<<
What then is a Harris hematoxylin? Traditionally the term "Harris" is used to
denote alum hematoxylins oxidized with mercuric oxide, and "Mayer" for those
oxidized with sodium iodate. (Gary Gill's formulations are Mayer
hematoxylins.)
It would appear that the present definition of Harris hematoxylin is "any
hematoxylin our Marketing Department wants called Harris hematoxylin"
Does it make a difference? I once encountered a situation where it probably
did. About thirty years ago (gack!) in setting up the Engel-Cunningham
modification of the Gomori trichrome stain for frozen sections of skeletal
muscle, I found that our homemade iodate hematoxylin didn't work, while a
sample of mercuric oxide hematoxylin purloined from the cytopathology
laboratory did. I added mercuric chloride to the iodate formula and it worked
fine.
Somehow "Richmond hematoxylin" never caught on.
Bob Richmond
Samurai Pathologist
P.S. "Questionnaire" is spelled with two n's. I am quite certain of this,
having won (and almost lost) the Grade School Spelling Championship of the
Archdiocese of San Antonio in 1951 (gack again) with that word.
How an Episcopalian got into that situation is another tale.
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