Re: [Histonet] endothelial cell staining

From:"Joanne Mauger"

To Jerry,
I have had good luck staining endothelium in mice with rat anti ms CD31 from Pharmingen(BD Biosciences) Catalog # is 557355.

>>> "Jeremiah Lyons"  07/23/04 11:23AM >>>

I'm a PhD student who is looking for advice on endothelial cell staining in mouse tumour tissue for the purpose of microvessel density staining.Most of the available antibodies don't work on mouse tissue. At the moment I'm trying Carnoy's fixed tissue (fixed for four hours) with rabbit anti-human Von Willebrand factor. Any suggestions would be greatly appreciated.

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