RE: [Histonet] PGP9.5 staining -- how to get rid of hair folliclebackground staining?
A couple of questions for you first: What is the thickness of your
sections? What concentration of primary do you normally use?
I do PGP 9.5 staining on 50 micron frozen sections of skin. The hair
follicles will stain somewhat but should not be overwhelming.
University of Wisconsin Hospital & Clinics
Department of Neurology
[mailto:firstname.lastname@example.org] On Behalf Of Cao,
Sent: Friday, July 23, 2004 8:02 AM
Subject: [Histonet] PGP9.5 staining -- how to get rid of hair
I am trying to stain for nerve fibers innervating the skin samples that
I have. While I am lucky to get the nerve fiber singnals, I am also
getting overwheelingly strong background staining from the hair
follicles. I know the follicle stains are background since they are also
stained in both of my controls where primary and secondary are omitted
are also stained. My question is: how to get rid of the hair follicle
stains? any suggestions? I am using Vector's ABC and SG substrate.
Xudong Cao, Ph.D.
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