[Histonet] Flk-1 immunostaining protocol

From:"Wael Swelam"

Dear all,
I have been trying immunostaining for Flk-1 using different protocoles (Autoclave treatment using citrate buffer OR Tris-EDTA), enzyme treatment (proteinase K 5ug/ml); for Flk-1 monoclonal antibody (Santa cruse) i used 1:50 conc using Envesion detecting system. but i don't have the strong specific signal.
Can any one help
Wael Swlam 
Ph.D student 
Niigata University,Japan
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