Hi Jennifer,

This is what i normally do when I overstained with
Eosin:

After rehydrating the specimen with Xylene and then
descending concentrations of ethanol;
 1. if the eosin is aqueous base, then i'll let the
slides for a few minutes in water until the intensity
of the Eosin is correct. 
2. If the Eosin is Alcoholic, then I’ll let the slides
in 95% Ethanol for a few minutes until the Eosin fade
to the intensity desired.

Jaimie
Research Officer
Singapore Eye Research Institute



	
	
		
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