Re: bone and IHC
i use zinc formalin fixation and 5% formic acid (Immunocal from Decal
Chemicals) decalcification and then routine paraffin processing for IHC
on bone samples. the zinc formalin will elliminate most need for
HIER which is really hard on bone sections. formic acid decal has
been shown to do the job without adversely affecting the antigen sites,
it is much faster than EDTA (which can affect proteoglycans) but slower
than HCL or Nitric decalcifiers which can be hard on antigen sites.
the sections tend to come off the slide easily unless you carefully drain
them before heating and of course good quality silane coated plus slides
are required. if you can avoid HIER it is suggested.
i prefer to use enzyme digestion EIER with bone IHC, I find it easier to
control. my preferred enzymes are proteinase k ready made from DAKO
and pepsin ready made from Research Genetics.
was wondering how people out there process bone samples that will be used
for immunohistochemistry - specifically rodent specimens. Any
insights would be most appreciated. And please try to cc me directly as
well as the Histonet. Thanks!Andrea
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