From:Darrell Panza

Would anyone know if there are any adverse affects of routine tissue processing on tissue (specifically mouse heart) containing CM-DiI (from Molecular Probes)?
I had thought that there may have been a problem with the ethanols, but had been told that this was not the case.
The mouse heart had been fixed with 4% paraformaldehyde
for 3-4 hours, kept in 30% sucrose solution for ~2 days
then processed:
70% ETOH-1hr.
80% ETOH-1hr.
90% ETOH-1.5hr.
100%ETOH-1.5hr (X3)
I have since cut and seen no DiI.
Thanks in advance for any suggestions,

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