Would anyone know if there are
any adverse affects of routine tissue processing on tissue
(specifically mouse heart) containing CM-DiI (from Molecular Probes)?
I had thought that there may have been a
problem with the ethanols, but had been told that this was not the
The mouse heart had been fixed with
for 3-4 hours, kept in 30% sucrose solution
for ~2 days
I have since cut and seen no DiI.
Thanks in advance for any suggestions,
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