Re: Does anyone have method to share???

From:Barbara Stancel

Dear Ian, I do not know anything about the Movat's, but we have used the 
PTAH (for muscle)from the old green AFIP book for many years. We made a 
liter of the PTAH solution in 1990. We let it ripen on its own for about 6 
months and it stains deeper and better every year that passes. Obviously we 
don't do a lot of PTAHs....maybe about five a year. We stain in very small 
slide mailers (the ones that hold five slides) so we only use about 20 mls 
at a time.  Good Luck.

Histologically yours,

Barbara H. Stancel, HTL(ASCP)HT
USDA, FSIS, OPHS, Eastern Laboratory, Pathology
RRC, 950 College Station Road
Athens, Georgia  30604
phone: (706) 546-3556
fax: (706) 546-3589

>From: Bernard Ian R SSgt 59 CRES/MSROP 
>To: "Histonet (E-mail)" 
>Subject: Does anyone have method to share???
>Date: Mon, 08 Jul 2002 09:12:01 -0500
>Does anyone have good methods on the Movat & PTAH stain to share.  We are
>looking for methods from folk that does these stains on a regular basis.
>We do this stain once every 2 years and for some reason it was too light
>overall.  We followed the new Carson's text method.  Any ideas??
>Ian R. Bernard, SSgt, USAF, AAS, HT (ASCP)
>NCOIC Histopathology/ Pathobiology Laboratory
>59th Clinical Research Sqd / MSROP
>1255 Wilford Hall Loop, bldg 4430
>Lackland AFB, Tx 78236-5319
>Tel: 210-292-7190
>Fax: 210-292-6053

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