> My suggestion is to use 5% decremented glycerols down to water.  Remember
> the danger from OSMOSIS grotes_cuss_us.
> 
> Your second question can be answered by successfully completing the
> process suggested in the answer given for the first question.
> 
> Repeat the original stain to see what, if anything, has been lost by
> glycerination.  
> 
> AND, are you studying muscle?
> 
> Hope this makes sense,
> 
> Fred Monson
> 
> Frederick C. Monson, PhD   
> Center for Advanced Scientific Imaging
> Schmucker II Science Center
> West Chester University
> South Church Street and Rosedale
> West Chester, Pennsylvania, USA, 19383
> Phone:  610-738-0437
> FAX:  610-738-0437
> fmonson@wcupa.edu
> CASI URL:  http://darwin.wcupa.edu/casi/
> WCUPA URL:  http://www.wcupa.edu/
> Visitors URL:  http://www.wcupa.edu/_visitors/
>   
> 
> ----------
> From: 	Debra Dunlap
> Sent: 	Monday, July 8, 2002 6:22 PM
> To: 	histonet@pathology.swmed.edu
> Subject: 	zebrafish stored in 75% glycerol
> 
> Hi,
>     We have some previously stained zebrafish which were stored in 75%
> glycerol.
> What is the best method to remove the glycerol in order perform immuno
> on the
> zebrafish?  Can a second round of immuno be performed on these stored
> samples?
> 
> Thank you in advance....deb
> 
> 
> 
> 
>