Re: sections loss after EDTA HIER
Hi,
Are you absolutely sure the sections are
completely dry before deparaffinizing them? A full hour of retrieval seems a bit
excessive. Perhaps a shorter retrieval time? Are you sure the sialinized slides
have been prepared properly? Have you tried another kind of slides?
Just a few thoughts to mull over,
Amos Brooks
----- Original Message -----
Sent: Monday, July 08, 2002 8:29 AM
Subject: sections loss after EDTA
HIER
Dear
Collegues,
I recently started to try out antigen
retrieval with 1mmol EDTA (pH 8.0, using steaming in a vegetable steamer for 1
hour). Unlike with steaming with citrate buffer pH 6.0, my sections were
completely lost after the EDTA treatment. We use double-silane coated
slides (Superfrost). Does anybody know why the sections are lost and whether
we have to take another kind of coating?
thanks in
advance
jan
-----------------------------------------------------
Jan Bauer,
Ph.D
Div. of Neuroimmunology
Brain Research Institute
Spitalgasse
4
A-1090
Vienna
Austria
tel: +43-1-4277
62813
fax: +43-1-4277 9628
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