Re: sections loss after EDTA HIER

From:Amos Brooks

    Are you absolutely sure the sections are completely dry before deparaffinizing them? A full hour of retrieval seems a bit excessive. Perhaps a shorter retrieval time? Are you sure the sialinized slides have been prepared properly? Have you tried another kind of slides?
Just a few thoughts to mull over,
Amos Brooks
----- Original Message -----
From: Jan Bauer
To: HistoNet Server
Sent: Monday, July 08, 2002 8:29 AM
Subject: sections loss after EDTA HIER

Dear Collegues,

I recently started to try out antigen retrieval with 1mmol EDTA (pH 8.0, using steaming in a vegetable steamer for 1 hour). Unlike with steaming with citrate buffer pH 6.0, my sections were completely lost after the EDTA treatment.  We use double-silane coated slides (Superfrost). Does anybody know why the sections are lost and whether we have to take another kind of coating?
thanks in advance

Jan Bauer, Ph.D
Div. of Neuroimmunology
Brain Research Institute
Spitalgasse 4
A-1090 Vienna

tel:    +43-1-4277 62813
fax:    +43-1-4277 9628

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