Re: sections loss after EDTA HIER

From:Amos Brooks

Hi,
    Are you absolutely sure the sections are completely dry before deparaffinizing them? A full hour of retrieval seems a bit excessive. Perhaps a shorter retrieval time? Are you sure the sialinized slides have been prepared properly? Have you tried another kind of slides?
Just a few thoughts to mull over,
Amos Brooks
----- Original Message -----
From: Jan Bauer
To: HistoNet Server
Sent: Monday, July 08, 2002 8:29 AM
Subject: sections loss after EDTA HIER

Dear Collegues,

I recently started to try out antigen retrieval with 1mmol EDTA (pH 8.0, using steaming in a vegetable steamer for 1 hour). Unlike with steaming with citrate buffer pH 6.0, my sections were completely lost after the EDTA treatment.  We use double-silane coated slides (Superfrost). Does anybody know why the sections are lost and whether we have to take another kind of coating?
thanks in advance
jan

-----------------------------------------------------
Jan Bauer, Ph.D
Div. of Neuroimmunology
Brain Research Institute
Spitalgasse 4
A-1090 Vienna
Austria

tel:    +43-1-4277 62813
fax:    +43-1-4277 9628


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