Dear Agustin:

Agustín Venzano wrote:

> Dear Netters: I'm needing two opinions from you:
>
> 1. What are your thoughts and experience on the use of chloroform as a
> clearing agent when processing nervous tissues?

I have had good results with it.

> 2. We are currently picking up brain sections on slides that haven't been
> previously coated with any adhesives. Some time ago we used Mayer's albumin,
> but we gave up this one due to its disgusting physical characteristics. What
> kind of adhesive substances are you presently using for routine H&E
> histopathology?

"Subbed" slides, ie.slides coated with a gelatin + chrome alum (chromium
potassium sulfate) are fine for routine stains and most immunos. However, high
temperatures, alkaline solutions or enzyme treatments may require "silane"
coated slides.

Geoff
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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