You forgot the phase of the moon dance and prayers histologist should do
every month.  If it is the wrong phase nothing works.  Pam Marcum

> -----Original Message-----
> From: rueggp [mailto:rueggp@earthlink.net]
> Sent: Monday, July 01, 2002 11:14 AM
> To: Nina Malja
> Cc: 'Tom McNemar'; 'Histonet mailinglist'
> Subject: Re: SV: Bone Marrow Iron Stain.
>
>
> or perhaps you forgot to pray to the gods of histotechnique
> patsy
>
> Nina Malja wrote:
>
> > It must have been The Lab Ghost... It visited us as well
> earlier this year.
> > The Lab Ghost then gave us troubles with DAB staining of bone
> marrow smears
> > and we never really figured it out. After checking out all our reagents,
> > temperatures, pH et cetera, we still didn't get any wiser...We
> even tried a
> > completely different methodology by using a DAKO kit. (The
> doctors didn't
> > like the result the kit gave so we couldn't use that either...)
> Now we are
> > back to were we started - with our old procedure working as it
> should again.
> > We are making our own giemsa stain now though. We think it
> might have been
> > wrong pH in the old giemsa we used, but that has never been
> verified. I bet
> > it was The Lab Ghost. Whenever something is wrong and we can't
> figure out
> > why - it's The Lab Ghost visiting again.  ;-)
> >
> > Have a wonderful summer all!!  :-)
> >
> > Nina Malja
> > Coagulation lab.
> > Rikshospitalet, Oslo
> > Norway
> >
> > Denne meldingen inneholder ikke sensitiv informasjon som bryter med
> > Rikshospitalets krav til informasjonssikkerhet.
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> >
> >
> >
> > -----Opprinnelig melding-----
> > Fra: Tom McNemar [mailto:TMcNemar@lmhealth.org]
> > Sendt: 1. juli 2002 12:26
> > Til: 'Baker, Christine'; histonet@pathology.swmed.edu
> > Emne: RE: Bone Marrow Iron Stain.
> >
> > We had the same problem... Never really resolved it.  We tried many
> > different reagents, a couple of different methods, and anything
> else I could
> > think of.  Even went back to making up our own reagents.  The
> control was
> > always beautiful.  We are now using Richard Allen's Chromoview (from
> > Allegiance) and are getting good results.
> >
> > I sure wish I knew what was going on.  We hadn't changed
> anything.... Had
> > been working fine for years.  I'll be interested to see other
> responses....
> >
> > Good luck!
> >
> > Tom Mc Nemar HT(ASCP)
> > Histology Supervisor
> > Licking Memorial Hospital
> > Newark, Ohio
>
>
>