RE: Daddi's Method

From:Roy Ellis

I have found a reference to Daddi's method in Lillie's 3rd Edition of
Histologic Technic and Practical Histochemistry. He states 'The original
method of Daddi (1896) called for a saturated solution of Sudan III in 70%
alcohol. This takes half an hour or more to stain, gives a rather light
orange color and dissolves out an appreciable amount of fat, in some
instances all of the demonstrable fat that was present.'
Roy Ellis

> -----Original Message-----
> From: Louri Caldwell []
> Sent: Thursday, 11 July 2002 01:22 AM
> To:
> Subject: Daddi's Method
> Hi everyone,
> I have a researcher here who would like to stain whole arteries
> with Sudan
> Black to demonstrate cholesterol.  He mentioned Daddi's Method,
> but I have
> been unable to find a reference for this -  Would any of you wonderful
> people have heard of such a thing?
> Any assistance you may provide is greatly appreciated.
> Louri Caldwell
> UGA College of Vet Med
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