RE: faded DAB
From: | "Monson, Frederick C." |
Tony,
If the DAB is still where it ought to be, would it be possible to
try the Nickel again? Or for the first time?
Just wondering,
Fred Monson
> ----------
> From: Tony Henwood
> Sent: Wednesday, July 10, 2002 6:59 PM
> To: 'Julie Clark'; HistoNet@pathology.swmed.edu
> Subject: RE: faded DAB
>
> Julie,
> I doubt whether it would be the brand of DAB. Dyes in slides will
> eventually fade over time. What we can do is keep the slides away from
> bright light (ie sunlight) and look at the mountant we are using. Some
> mountants retard fading better than others. In my experience this is often
> the major cause of early fading of stained slides in my opinion.
>
> To bring back the brown colour, you could try osmicating decoverslipped
> sections (Beware osmium tetroxide is dangerous stuff). This has worked for
> me about 3 of 10 times I have tried it.
>
> Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager
> The Children's Hospital at Westmead,
> Locked Bag 4001, Westmead, 2145, AUSTRALIA.
> Tel: (02) 9845 3306
> Fax: (02) 9845 3318
>
> http://www.histosearch.com/homepages/TonyHenwood/default.html
> http://us.geocities.com/tonyhenwoodau/index.html
>
>
> -----Original Message-----
> From: Julie Clark [ mailto:jclark@kumc.edu]
> Sent: Wednesday, 10 July 2002 22:55
> To: HistoNet@pathology.swmed.edu
> Subject: faded DAB
>
>
> I have been asked to review some two year-old immuno slides. Most of the
> slides are very faded to completely faded. I have no idea what brand DAB
> they were using.
>
> Has anyone tried to re-stain previously DAB stained immuno slides? I have
> to use DAB, I cannot switch chromogens.
>
> Is there some way to remove the faded DAB?
>
> Thanks,
>
> Julie Clark
> Technical Director
> Breast Cancer Prevention Center Research Laboratory
> Room 3021 Lied
> Univ. of Kansas Med. Center
> Kansas City, KS
> Phone: Office: 913-588-3963
> Lab: 913-588-3917
> FAX: 913-588-3821
> Page: 913-917-6052
>
>
>
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