Dear Netters, I am staining animal tissues cow , cat, goat and want to increase the staining of the smooth muscle. I use 2% eosin y (alcoholic) for 2 min 30 sec, 30 sec in 95% ethanol, then dehydrate to xylene. Nuclear stain is Gill III for 6 minutes. Any suggestions would be greatly appreciated. Also, will there be a histonet gathering at the NSH this year? Many thanks Pat HTN inc.