Re: CD117

From:Amos Brooks

    Use the Dako antibody! We get good results with no pretreatment at 1:200
with LSAB+ detection. Use colon as a control as it is usually mast cell
rich. Our results have been very reliable.
Amos Brooks

----- Original Message -----
From: "Krahn, Daniel" 
Sent: Friday, July 27, 2001 11:03 AM
Subject: CD117

> Hi there,
> Could anyone please help out with a protocol for running CD117?  We
> have been validating the Santa Cruz polyclonal (goat) and the Dako
> polyclonal (rabbit) and are having some difficulties getting consistent
> results.
> A test protocol would be very helpful, including method of antigen
> retrieval, dilution factors, instrumentation, et

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