Dear Linda,

Again I believe Mary Stevens has given good advice.  If the specimens are
in normal sized tissue processing cassettes, how ever many specimens can be
covered say with 100 ml of fluid is good (probably 5-8).  Agitaing the
fluid or putting the container under vacuum will also aid in the
infiltration of the monomer.  I don't believe it is like fixation, where
there needs to be a certain ratio of monomer to tissue.  I believe it is
important to have at least 2 changes prior to embedding in freshly made
solution.

Regards,
Cathy


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GLP Compliant Laboratory

Cathy A. Mayton
Wasatch Histo Consultants, Inc.
www.wasatchhisto.com