Re: ER and Golgi

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From:"J. A. Kiernan" <>
To:Kenneth Chapman <>
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On Mon, 31 Jul 2000, Kenneth Chapman wrote:

> Hi John,
> Thanks for answering!  You did this staining on paraffin sections?  Do you
> have any information regarding protocols?
> Ken

  Yes, on paraffin sections after fixation in (if I remember rightly)
  both neutral buffered formaldehyde and Carnoy (on different blocks,
  that is!) If you let me know your mailing address I can send you
  two reprints. One is by R. W. Stoddart and me on staining with
  fluorescent concanavalin A (I think it's the first published
  description of fluorescent lectin staining of sections; other
  people had used them on cells). The other describes a sandwich
  method using con A and HRP. 

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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