Re: Nerve staining for human fetuses
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From: | Gayle Callis <uvsgc@msu.oscs.montana.edu> |
To: | gjohnson@gandalf.otago.ac.nz, histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
I would avoid using a strong acid decalcifier aka HCl or nitric, and it may
take less decalcification, depending on the age of the fetus. A buffered
formic acid would be acceptable, and EDTA would be even better IF you have
time to wait for
EDTA to decalcify. I would also do endpoint determinations when using
formic acid to insure your precious spines are not overexposed to acid, and
ruin the needed immunostaining.
At 09:22 AM 7/4/00 +1200, you wrote:
>I have some precious human fetal tissue (fixed in formaldehyde)
>and I'm interested in doing some staining before I start immuno
>work. I'm working on the spine so the sections will need to be
>decalicifed beforehand.
>Any comments most welcome
>Gill
>
>
>
Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303
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