RE: Frozen sectioning

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From:Cynthia Favara <cfavara@niaid.nih.gov>
To:'Scott McDonald' <smcdonld@med.usyd.edu.au>, HistoNet Server <histonet@pathology.swmed.edu>
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Scott,
	Tissue-Tek makes cryomold and you can make a nice block surrounded
by the OCT. There has been a lot of disscussion as to the content of OCT and
I have ignored most of it as it is irrelevant to what I do. I have cut thick
sections up to 50um of mouse brain and the major problem is cracking. I have
found I have to increase the temperature for thicker sections. I might be of
more help if I knew what was going to be done with the tissue after you cut.

Cynthia Favara 
NIAID/RML/LPVD 
903 South 4th Street 
Hamilton, MT 59840 
PH: 406-363-9317 
FAX: 406-363-9286 

e-mail: CF98d@nih.gov <mailto:CF98d@nih.gov> 

 




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