Geoff,

Where do you purchase DPX and would you be able to supply a catalog
number and cost?

Thanks,

Hermina Borgerink
Wake Forest University
Department of Pathology
Winston-Salem, NC

Geoff McAuliffe wrote:
> 
> vedran lovic wrote:
> 
> > Hi everyone,
> >           Several months ago we've used KPL's True Blue instead of DAB in an
> > immunocytochemistry (ICC) run for the first time. We were staining GnRH
> > neurons in bird brains.  At the time the procedure worked fine, but I just
> > went back to quantify some neurons in these same brains and it seems that
> > there has been a drastic fading of True Blue neuronal staining.  Does anyone
> > have any experience with True Blue?  Any input would be greatly appreciated.
> >   Thanx.
> >
> > Ved Lovic
> > University of Toronto at Mississauga
> > 3359 Mississauga Rd. N. #1029e
> > Mississauga, ON
> > L5L 1C6
> > (905)828-5297
> > vedranl@hotmail.com
> >
> 
>     My experience is that DAB, even with intensification with nickel and cobalt,
> will fade if Permount is the coverslip mounting medium. I don't know about True
> Blue. I now use only DPX to mount coverslips.
> 
> Geoff
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029
> mcauliff@umdnj.edu
> **********************************************